Citation link: http://dx.doi.org/10.25819/ubsi/9920
DC FieldValueLanguage
crisitem.author.orcid0000-0002-2392-851X-
crisitem.author.orcid0000-0002-9186-5565-
crisitem.author.orcid0000-0002-6023-4381-
dc.contributor.authorHartmann, Sarah-
dc.contributor.authorKunze, Jan-
dc.contributor.authorVogt, Roland-
dc.contributor.authorRauschert, Anna-
dc.contributor.authorKuhnert, Klaus-Dieter-
dc.contributor.authorWanzenböck, Josef-
dc.contributor.authorLamatsch, Dunja K-
dc.contributor.authorWitte, Klaudia-
dc.date.accessioned2021-06-14T13:41:37Z-
dc.date.available2021-06-14T13:41:37Z-
dc.date.issued2018de
dc.descriptionFinanziert aus dem DFG-geförderten Open-Access-Publikationsfonds der Universität Siegen für Zeitschriftenartikelde
dc.description.abstractZebrafish larvae (Danio rerio) are among the most used model species to test biological effects of different substances in biomedical research, neuroscience and ecotoxicology. Most tests are based on changes in swimming activity of zebrafish larvae by using commercially available high-throughput screening systems. These systems record and analyse behaviour patterns using visible (VIS) and near-infrared (NIR) light sources, to simulate day (VIS) and night (NIR) phases, which allow continuous recording of the behaviour using a NIR sensitive camera. So far, however, the sensitivity of zebrafish larvae to NIR has never been tested experimentally, although being a critical piece of information for interpreting their behaviour under experimental conditions. Here, we investigated the swimming activity of 96 hpf (hours post fertilization) and 120 hpf zebrafish larvae under light sources of NIR at 860 nm and at 960 nm wavelength and under VIS light. A thermal source was simultaneously presented opposite to one of the light sources as control. We found that zebrafish larvae of both larval stages showed a clear negative phototactic response towards 860 nm NIR light and to VIS light, but not to 960 nm NIR light. Our results demonstrated that zebrafish larvae are able to perceive NIR at 860 nm, which is almost identical to the most commonly used light source in commercial screening systems (NIR at 850 nm) to create a dark environment. These tests, however, are not performed in the dark from the zebrafish´s point of view. We recommend testing sensitivity of the used test organism before assuming no interaction with the applied light source of commonly used biosensor test systems. Previous studies on biological effects of substances to zebrafish larvae should be interpreted with caution.en
dc.identifier.doihttp://dx.doi.org/10.25819/ubsi/9920-
dc.identifier.urihttps://dspace.ub.uni-siegen.de/handle/ubsi/1909-
dc.identifier.urnurn:nbn:de:hbz:467-19090-
dc.language.isoende
dc.sourcePLoS ONE ; 13 (11), e0207264. - https://doi.org/10.1371/journal.pone.0207264de
dc.subject.ddc590 Tiere (Zoologie)de
dc.subject.otherZebrafishen
dc.subject.otherPhototaxisen
dc.subject.swbZebrabärblingde
dc.subject.swbPhototaxisde
dc.titleZebrafish larvae show negative phototaxis to near-infrared lighten
dc.typeArticlede
item.fulltextWith Fulltext-
ubsi.publication.affiliationInstitut für Biologiede
ubsi.source.authorPublic Library of Sciencesde
ubsi.source.doi10.1371/journal.pone.0207264-
ubsi.source.issn1932-6203-
ubsi.source.issued2018de
ubsi.source.issuenumber11de
ubsi.source.linkhttps://journals.plos.org/plosone/de
ubsi.source.pages16de
ubsi.source.publisherPublic Library of Sciencesde
ubsi.source.titlePLoS ONEde
ubsi.source.volume13de
ubsi.subject.ghbsVOAde
ubsi.subject.ghbsVQSde
ubsi.subject.ghbsVSCde
Appears in Collections:Geförderte Open-Access-Publikationen
Files in This Item:
File Description SizeFormat
Zebrafish_larvae_show_negative_phototaxis.pdf1.1 MBAdobe PDFThumbnail
View/Open

This item is protected by original copyright

Show simple item record

Page view(s)

254
checked on Mar 29, 2024

Download(s)

40
checked on Mar 29, 2024

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.