Citation link: http://dx.doi.org/10.25819/ubsi/10146
DC FieldValueLanguage
crisitem.author.orcid0000-0002-9744-3419-
crisitem.author.orcid0000-0002-5836-5569-
crisitem.author.orcid0000-0003-3282-5982-
dc.contributor.authorSoltani, Navid-
dc.contributor.authorRabbany Esfahany, Elham-
dc.contributor.authorDruzhinin, Sergey I.-
dc.contributor.authorSchulte, Gregor-
dc.contributor.authorMüller, Julian-
dc.contributor.authorButz, Benjamin-
dc.contributor.authorSchönherr, Holger-
dc.contributor.authorAgio, Mario-
dc.contributor.authorMarkešević, Nemanja-
dc.date.accessioned2022-11-02T12:10:41Z-
dc.date.available2022-11-02T12:10:41Z-
dc.date.issued2022de
dc.descriptionFinanziert aus dem Open-Access-Publikationsfonds der Universität Siegen für Zeitschriftenartikelde
dc.description.abstractWe investigate a model bioassay in a liquid environment using a z-scanning planar Yagi-Uda antenna, focusing on the fluorescence collection enhancement of ATTO-647N dye conjugated to DNA (deoxyribonucleic acid) molecules. The antenna changes the excitation and the decay rates and, more importantly, the emission pattern of ATTO-647N, resulting in a narrow emission angle (41°) and improved collection efficiency. We efficiently detect immobilized fluorescently-labeled DNA molecules, originating from solutions with DNA concentrations down to 1 nM. In practice, this corresponds to an ensemble of fewer than 10 ATTO-647N labeled DNA molecules in the focal area. Even though we use only one type of biomolecule and one immobilization technique to establish the procedure, our method is versatile and applicable to any immobilized, dye-labeled biomolecule in a transparent solid, air, or liquid environment.en
dc.identifier.doihttp://dx.doi.org/10.25819/ubsi/10146-
dc.identifier.urihttps://dspace.ub.uni-siegen.de/handle/ubsi/2234-
dc.identifier.urnurn:nbn:de:hbz:467-22343-
dc.language.isoende
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceBiomedical Optics Express, 13 (2), S. 539-548, 2022. - https://doi.org/10.1364/BOE.445402de
dc.subject.ddc530 Physikde
dc.subject.otherBiosensingen
dc.subject.otherYagi-Uda antennaen
dc.subject.otherYagi-Antennede
dc.subject.swbRichtstrahlerde
dc.subject.swbBiosensorde
dc.subject.swbFluoreszenzde
dc.titleBiosensing with a scanning planar Yagi-Uda antennaen
dc.typeArticlede
item.fulltextWith Fulltext-
ubsi.publication.affiliationDepartment Physikde
ubsi.source.issn2156-7085-
ubsi.source.issued2022de
ubsi.source.issuenumber2de
ubsi.source.pagefrom539de
ubsi.source.pageto548de
ubsi.source.publisherOptica Publishing Groupde
ubsi.source.titleBiomedical Optics Expressde
ubsi.source.volume13de
ubsi.subject.ghbsUKSde
ubsi.subject.ghbsURDde
ubsi.subject.ghbsYDDde
Appears in Collections:Publikationen aus der Universität Siegen
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