Citation link: http://dx.doi.org/10.25819/ubsi/10146
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Dokument Type: Article
metadata.dc.title: Biosensing with a scanning planar Yagi-Uda antenna
Authors: Soltani, Navid 
Rabbany Esfahany, Elham 
Druzhinin, Sergey I. 
Schulte, Gregor 
Müller, Julian 
Butz, Benjamin 
Schönherr, Holger 
Agio, Mario 
Markešević, Nemanja 
Institute: Department Physik 
Free keywords: Biosensing, Yagi-Uda antenna, Yagi-Antenne
Dewey Decimal Classification: 530 Physik
GHBS-Clases: UKS
URD
YDD
Issue Date: 2022
Publish Date: 2022
Source: Biomedical Optics Express, 13 (2), S. 539-548, 2022. - https://doi.org/10.1364/BOE.445402
Abstract: 
We investigate a model bioassay in a liquid environment using a z-scanning planar Yagi-Uda antenna, focusing on the fluorescence collection enhancement of ATTO-647N dye conjugated to DNA (deoxyribonucleic acid) molecules. The antenna changes the excitation and the decay rates and, more importantly, the emission pattern of ATTO-647N, resulting in a narrow emission angle (41°) and improved collection efficiency. We efficiently detect immobilized fluorescently-labeled DNA molecules, originating from solutions with DNA concentrations down to 1 nM. In practice, this corresponds to an ensemble of fewer than 10 ATTO-647N labeled DNA molecules in the focal area. Even though we use only one type of biomolecule and one immobilization technique to establish the procedure, our method is versatile and applicable to any immobilized, dye-labeled biomolecule in a transparent solid, air, or liquid environment.
Description: 
Finanziert aus dem Open-Access-Publikationsfonds der Universität Siegen für Zeitschriftenartikel
DOI: http://dx.doi.org/10.25819/ubsi/10146
URN: urn:nbn:de:hbz:467-22343
URI: https://dspace.ub.uni-siegen.de/handle/ubsi/2234
License: http://creativecommons.org/licenses/by-nc-nd/4.0/
Appears in Collections:Publikationen aus der Universität Siegen

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