Citation Link: https://doi.org/10.25819/ubsi/10249
Opening opportunities for Kd determination and screening of MHC peptide complexes
Source Type
Article
Issue Date
2022
Abstract
An essential element of adaptive immunity is selective binding of peptide antigens by major histocompatibility complex (MHC) class I proteins and their presentation to cytotoxic T lymphocytes. Using native mass spectrometry, we analyze the binding of peptides to an empty disulfide-stabilized HLA-A*02:01 molecule and, due to its unique stability, we determine binding affinities of complexes loaded with truncated or charge-reduced peptides. We find that the two anchor positions can be stabilized independently, and we further analyze the contribution of additional amino acid positions to the binding strength. As a complement to computational prediction tools, our method estimates binding strength of even low-affinity peptides to MHC class I complexes quickly and efficiently. It has huge potential to eliminate binding affinity biases and thus accelerate drug discovery in infectious diseases, autoimmunity, vaccine design, and cancer immunotherapy.
Description
Finanziert im Rahmen der DEAL-Verträge durch die Universitätsbibliothek Siegen
File(s)![Thumbnail Image]()
Loading...
Name
Opening_opportunities.pdf
Size
1.25 MB
Format
Adobe PDF
Checksum
(MD5):271bbe7c2a7647c4385bc52a68405286
Owning collection