Schulte, Anna
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Publication Access status: Metadata only , Anodic Aluminum Oxide Membrane Assisted Extrusion of Polymer Solutions for Hydrogel FormationThe potential of polymeric nanostructures obtained by extruding polymer solutions through reusable anodic aluminum oxide (AAO) nanopores for the fabrication of nanostructures is investigated systematically to be able to unveil the potential impact of structure on cell culturing. Thus, nanostructured Na‐alginate and poly(vinyl alcohol) (PVA) polymers as well as blended alginate/PVA hydrogels are fabricated. Na‐alginate and PVA fibers with different dimensions can be obtained. By extruding Na‐alginate solutions into a non‐solvent also networks of uniform nanofibers (diameter <20 nm) are generated. PVA forms agglomerations of nanofibers and nanopArticles with diameter >100 nm. In ADDition, cell line‐dependent behavior of the cross‐linked PVA/alginate nanofibers is observed, which is characterized by reduced cell adhesion and cluster formation of PaTu 8988t cells on these blend nanofibers compared to normal cell adhesive behavior of NIH 3T3 cells.Source Type:Article4 - Some of the metrics are blocked by yourconsent settings
Publication Access status: Metadata only , On‐Demand Cell Sheet Release with Low Density Peptide‐Functionalized Non‐LCST Polymer BrushesCell sheet harvesting offers a great potential for the development of new therapies for regenerative medicine. For cells to adhere onto surfaces, proliferate, and to be released on demand, thermoresponsive polymeric coatings are generally considered to be required. Herein, an alternative approach for the cell sheet harvesting and rapid release on demand is reported, circumventing the use of thermoresponsive materials. This approach is based on the end‐group biofunctionalization of non‐thermoresponsive and antifouling poly(2‐hydroxyethyl methacrylate) (p(HEMA)) brushes with cell‐adhesive peptide motifs. While the nonfunctionalized p(HEMA) surfaces are cell‐repellant, ligation of cell‐signaling ligand enables extensive attachment and proliferation of NIH 3T3 fibroblasts until the formation of a confluent cell layer. Remarkably, the formed cell sheets can be released from the surfaces by gentle rinsing with cell‐culture medium. The release of the cells is found to be facilitated by low surface density of cell‐adhesive peptides, as confirmed by X‐ray photoelectron spectroscopy. Additionally, the developed system affords possibility for repeated cell seeding, proliferation, and release on previously used substrates without any additional pretreatment steps. This new approach represents an alternative to thermally triggered cell‐sheet harvesting platforms, offering possibility of capture and proliferation of various rare cell lines via appropriate selection of the cell‐adhesive ligand.Source Type:Article4
