Fluoreszenzspektroskopie als neue Meßmethode zur höchstempfindlichen Untersuchungen transienter Zustände

The aim oft the present work was to extend the range of application of the existing transient
spectrometer by establishing the so called rotational-cuvette-technique. Thereby the basic
measurement system had disadvantages. Those disadvantages should be removed by the
development of a new principle of measurement to carry out measurements on fluorescent
dyes applied in actual scientific investigations.
In the first part of the work the development of the transient spectrometer is shown, giving the
possibility to detect the triplet lifetime an the intersystem crossing rate constant of organic
dyes by absorption measurements.
In the second part new investigations of transient states of various fluorescent dyes are
presented, enabled by the further development of the transient spectrometer.
In the third part the idea of fluorescent reduction has been picked up and a new principle of
measurement has been introduced, based on the detection of fluorescence. By the application
of this new principle it was possible to carry out measurements, which were not possible by
using the spectrometer based on the measurement of absorption. The results of these
investigations on fluorescent dyes applied in actual scientific research are shown in the fourth
part of the present work.
The combination of the absorption- and the fluorescence-based measurement system in
combination with the new rotational-cuvette-system offers a measurement equipment with a
widely enlarged range of application.